Function and distribution of of miRNA in pituitary adenoma

本研究旨在探讨垂体腺瘤细胞中通过miRNA调控催乳素（PRL）的信号级联反应。比较大鼠正常垂体（RPC）和大鼠垂体腺瘤细胞系GH 3的谱分析，以鉴定显示负相关的miRNA。随后，通过靶向siRNA敲低每种miRNA，然后进行ELISA研究以评估PRL分泌。此外，通过cDNA微阵列分析来分析信号级联。在GH 3细胞中显著高于RPC的miRNA中，我们发现rno-miR-101 b、-191和-194与PRL分泌有关。同时，转染rno-miR-101 b基因的GH 3细胞显示PRL及其受体的表达降低。总之，GH 3表达不同量的miRNA，特别是rno-miR-101介导的PRL产生。该miRNA有望成为垂体腺瘤治疗的新靶点。

In the present study, we focused on signaling cascade via miRNA regulating prolactin(PRL) in pituitary adenoma cell. Profiling analysis comparing rat normal pituitary(RPC) and GH3, rat pituitary adenoma cell line, was examined to identify miRNA showing negative co-relation. Subsequently, each miRNA was knocked-down by targeting siRNA followed by ELISA study to evaluate PRL secretion. Furthermore, signaling cascade was analyzed by cDNA microarray analysis. Among miRNA significantly elevated in GH3 cells than RPC, we found rno-miR-101b,-191, and-194 were related to PRL secretion. Meanwhile, GH3 cells transfected of rno-miR-101b gene revealed reduced expression of PRL and its receptor. In conclusion, GH3 expressed different amount of miRNA and in particular, rno-miR-101 mediated PRL production. The miRNA can be a promising target for the treatment of pituitary adenoma.