Leneage analysis of postnatal neurogenesis in organotypic culture of hippocampus

使用逆转录病毒载体编码的绿色荧光蛋白，我们标记增殖细胞的器官型切片培养的出生后大鼠海马和跟踪的绿色荧光蛋白标记的单一祖细胞和它的后代作为一个谱系在很长一段时间。我们发现新生成的神经元经常变得不可追踪，这个关键的可追踪期是2-14天。结果表明，新生神经元存活超过临界期后，可分化为成熟的齿状颗粒神经元。我们还创建了新的遗传探针，其由光遗传学或化学致动器和凋亡检测报告子组成。它们将帮助我们揭示以活动依赖方式决定新生成神经元命运的过程。

Using the retrovirus vectors encoding EGFP, we labeled proliferating cells in an organotypic slice culture of the postnatal rat hippocampus and tracked an EGFP-labeled single progenitor cell and its descendants as a lineage over a long period. We found that the newly generated neurons became frequently untraceable and this critical traceability period was 2-14 days. It is suggested that the newly generated neurons differentiate into mature dentate granule neurons once they have survived over the critical period. We also created novel genetic probes, which consist of an optogenetic or chemical actuator and an apoptosis detecting reporter. They would help us to reveal the processes that determine the fate of newly generated neurons in an activity-dependent manner.