Analysis of dynamic interaction between dopamine receptors and calcium channels located on the synaptic terminals in the basal forebrain

本电生理学研究阐明了多巴胺D1样受体介导的多巴胺能传递到胆碱能神经元的抑制作用在大鼠基底前脑通过腺苷酸环化酶和P/Q型钙通道调节的发育增加。本电化学研究，引入一种新的生物传感器材料，碳纳米管，已成功地检测生理释放的多巴胺作为脑切片制备的氧化还原电流。此外，本研究已经建立了一种方法，同时记录多巴胺释放和膜电位，从而阐明D2样受体的激活减少多巴胺释放的超极化多巴胺能神经元的膜。

The present electrophysiological study has clarified a developmental increase in dopamine D1-like receptor-mediated inhibition of glutamatergic transmission onto cholinergic neurons in the rat basal forebrain through adenylyl cyclase and P/Q-type calcium channel regulation.The present electrochemical study, introducing a new biosensor material, carbon nanotube, has succeeded in the detection of physiologically released dopamine as a redox current in brain slice preparation. Furthermore, the present study has established a method for simultaneous recordings of dopamine release and membrane potential, thereby clarifying that activation of D2-like receptors reduces dopamine release by hyperpolarizing the membrane of dopaminergic neurons.