Analysis of dynamic interaction between dopamine receptors and calcium channels located on the synaptic terminals in the basal forebrain

本电生理研究明确了多巴胺d1样受体通过腺苷酸环化酶和P/ q型钙通道调节介导的谷氨酸能传递到大鼠基底前脑胆碱能神经元的发育增加。本电化学研究引入了一种新的生物传感器材料——碳纳米管，成功地检测了脑切片制备过程中生理性释放的多巴胺作为氧化还原电流。此外，本研究建立了一种同时记录多巴胺释放和膜电位的方法，从而阐明了d2样受体的激活通过多巴胺能神经元膜的超极化来减少多巴胺释放。

The present electrophysiological study has clarified a developmental increase in dopamine D1-like receptor-mediated inhibition of glutamatergic transmission onto cholinergic neurons in the rat basal forebrain through adenylyl cyclase and P/Q-type calcium channel regulation.The present electrochemical study, introducing a new biosensor material, carbon nanotube, has succeeded in the detection of physiologically released dopamine as a redox current in brain slice preparation. Furthermore, the present study has established a method for simultaneous recordings of dopamine release and membrane potential, thereby clarifying that activation of D2-like receptors reduces dopamine release by hyperpolarizing the membrane of dopaminergic neurons.