Identification of tyrosine-sulfated proteins essential for sperm-egg interaction

酪氨酸（Tyr）硫酸化是一种常见的分泌蛋白或膜结合蛋白的翻译后修饰，涉及许多生理和病理过程。有趣的是，tpst2突变体由于精子运输、获能和/或受精异常而表现出严重的甲状腺功能减退和男性不育。这些结果表明未知的睾丸特异性蛋白(s)优先被TPST2硫酸酸化。在这项研究中，我试图鉴定对精子-卵子粘附至关重要的酪氨酸硫酸盐蛋白。

Tyrosine (Tyr) sulfation is a common posttranslational modification of secreted proteins or membrane-bound proteins that is implicated in numerous physiological and pathological processes. Interestingly, the Tpst2mutant shows severe hypothyroidism and male infertility due to the abnormality of sperm transport and capacitation and /or fertilization. These results suggest that the unknown testis specific protein(s) are preferentially sulfated by TPST2. In this study, I tried to identify Tyr-sulfated-protein(s) essential for sperm-egg adhesion.