Development of cell microarray as devices for cell diagnosis, regulation of cell function, and recovery of target cells

以聚N-异丙基丙烯酰胺(PNiPAAm)或N-琥珀酰亚胺基丙烯酸甲酯/N-琥珀酰亚胺(MA)为表面引发聚合反应，制备了4种微阵(MA：孔径分别为20、30、40、50μm；螺距为100μm)与抗体特异性结合的聚N-异丙基丙烯酰胺或生物相容性微球。聚(MPC)被涂覆在MA上的斑点周围。以HL60细胞或诱导人多能干细胞为典型细胞，与抗体反应后，作为靶向细胞的特异性结合部位，实现选择性细胞贴壁。利用研制的设备还成功地对MA上附着的细胞进行了高通量诊断。通过降低细胞培养温度，90%以上的HeLa细胞附着在PNiPAAm包被的MA斑点上。从37℃到25℃。含有人类人工染色体(HAc)的微细胞(MC)S与白细胞U937在固定化MA上的融合率高于悬浮细胞融合率(0.001%)，最高融合率为0.1%。作为细胞诊断、细胞功能调节和靶细胞恢复的工具，MA是有用的和实用的。

Four types of microarrays(MA : well sizes=20, 30, 40, 50μm ; pitch=100μm) spotted with poly(N-isopropylacrylamide ; PNiPAAm) or biocompatible MPC polymers having anti-body as a specific binding for target cells were prepared by surface-initiated polymerization of NiPAAm or MPC/N-succinimidylacrylate on the glass surface of MA. Poly(MPC) was coated around spots on the MAs. Selective cell attachment was achieved by using HL60 cells or induced human pluripotent stem(iPS) cells as a typical cell on the MAs after being reacted with anti-body as a specific binding site for targeting cells. High-throughput diagnosis of attached cells on the MA was also succeeded by using developed equipments. Over 90% attached HeLa cells on the PNiPAAm coated spots of the MA were efficiently exfoliated by decreasing cell cultured temp. from 37℃to 25℃. Microcell(MC) s containing Human Artifical Chromosome(HAC) and U937 as white blood cell were higher fused on the MCs immobilized MA with a maximum of 0.1% than that of suspension cell fusion(0.001%). The MAs were of useful and for practical uses as devices for cell diagnosis, regulation of cell function, and recovery of target cells.