Development of cell microarray as devices for cell diagnosis, regulation of cell function, and recovery of target cells

采用NiPAAm或MPC/ n -琥珀酰丙烯酸酯在MA的玻璃表面表面引发聚合的方法，制备了四种类型的微阵列（MA：孔尺寸=20、30、40、50μm，间距=100μm），这些微阵列中含有聚（n -异丙基丙烯酰胺；PNiPAAm）或具有抗体特异性结合靶细胞的生物相容性MPC聚合物。在MAs上的斑点周围涂覆Poly（MPC）。将HL60细胞或诱导的人多能干细胞（iPS）细胞作为典型细胞与抗体反应后，作为靶向细胞的特异性结合位点，在MAs上实现选择性细胞附着。利用先进的仪器，成功地对附着细胞进行了高通量诊断。将细胞培养温度从37℃降低到25℃，PNiPAAm包被点上90%以上的HeLa细胞能有效脱落。含有人人工染色体（Human Artifical Chromosome， HAC）和白细胞U937的微细胞（MCs）在MCs固定的MA上的融合率最高为0.1%，高于悬浮细胞的融合率（0.001%）。MAs作为细胞诊断、细胞功能调节和靶细胞恢复的设备具有实用价值。

Four types of microarrays(MA : well sizes=20, 30, 40, 50μm ; pitch=100μm) spotted with poly(N-isopropylacrylamide ; PNiPAAm) or biocompatible MPC polymers having anti-body as a specific binding for target cells were prepared by surface-initiated polymerization of NiPAAm or MPC/N-succinimidylacrylate on the glass surface of MA. Poly(MPC) was coated around spots on the MAs. Selective cell attachment was achieved by using HL60 cells or induced human pluripotent stem(iPS) cells as a typical cell on the MAs after being reacted with anti-body as a specific binding site for targeting cells. High-throughput diagnosis of attached cells on the MA was also succeeded by using developed equipments. Over 90% attached HeLa cells on the PNiPAAm coated spots of the MA were efficiently exfoliated by decreasing cell cultured temp. from 37℃to 25℃. Microcell(MC) s containing Human Artifical Chromosome(HAC) and U937 as white blood cell were higher fused on the MCs immobilized MA with a maximum of 0.1% than that of suspension cell fusion(0.001%). The MAs were of useful and for practical uses as devices for cell diagnosis, regulation of cell function, and recovery of target cells.