The analysis of protein dynamics by using NMR with induced laser

诱导激光核磁共振分析蛋白质动力学

• 批准号: 21740230
• 负责人: HAMADA Norio
• 金额: $ 2.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21740230/
• 关键词: 光物性; NMR; レーザー; 分光; 構造変化

We developed NMR system installed laser light at Osaka university. This NMR system was characterinzed the properties of molecules such as light-switching and light-active ones. In the research of Photoactive Yellow Protein(PYP, blue light photoreceptor protein) active-center model compounds, we reported that the intramolecular hydrogen bonds played an important role in its photoreaction and color regulation. In the investigations of light-switching molecules, we have assigned the signals related to light-induced conformational changes from obtained NMR data. Based on these results, we tried to apply the measurement of photoreceptor protein in order to research light-induced conformational changes. We also planned to install infrared laser lights into the NMR equipment in order to analyze the protein dynamics by inducing the perturbation of specified vibrational modes with IR laser. However, in this time, the light-induced conformational changes of photoreceptor protein were not able to be analyzed by this system and we couldn' t develop the proper IR laser and installation system. Now we have continued to measure photoreceptor proteins and analyzed data.

我们在大坂大学开发了安装激光的NMR系统。利用该核磁共振系统对分子的光开关和光活性等性质进行了表征。在光敏黄蛋白（PYP，blue light photoreceptor protein）活性中心模型化合物的研究中，我们发现分子内氢键在其光反应和颜色调控中起着重要作用。在光开关分子的研究中，我们已经从获得的NMR数据中分配了与光诱导构象变化相关的信号。在此基础上，我们尝试将光感受器蛋白的测量应用于光诱导构象变化的研究。我们还计划在核磁共振设备中安装红外激光，以便通过红外激光诱导特定振动模式的扰动来分析蛋白质动力学。然而，目前该系统还不能分析光诱导的光感受器蛋白构象变化，也不能开发合适的红外激光器和安装系统。现在我们继续测量感光蛋白并分析数据。