The analysis of protein dynamics by using NMR with induced laser

我们在大阪大学开发了安装激光的核磁共振系统。该核磁共振系统表征了分子的光开关和光活性等性质。在光活性黄蛋白(PYP，蓝光感光蛋白)活性中心模型化合物的研究中，我们报道了分子内氢键在其光反应和颜色调节中起着重要作用。在光开关分子的研究中，我们从获得的核磁共振数据中指定了与光诱导构象变化有关的信号。基于这些结果，我们尝试将光感受器蛋白的测量应用于研究光诱导的构象变化。我们还计划在核磁共振设备中安装红外激光，以便通过红外激光诱导特定振动模式的扰动来分析蛋白质动力学。然而，在这一时期，光感受器蛋白的光诱导构象变化无法用该系统来分析，我们也无法开发出合适的红外激光器和安装系统。现在我们继续测量光感受器蛋白并分析数据。

We developed NMR system installed laser light at Osaka university. This NMR system was characterinzed the properties of molecules such as light-switching and light-active ones. In the research of Photoactive Yellow Protein(PYP, blue light photoreceptor protein) active-center model compounds, we reported that the intramolecular hydrogen bonds played an important role in its photoreaction and color regulation. In the investigations of light-switching molecules, we have assigned the signals related to light-induced conformational changes from obtained NMR data. Based on these results, we tried to apply the measurement of photoreceptor protein in order to research light-induced conformational changes. We also planned to install infrared laser lights into the NMR equipment in order to analyze the protein dynamics by inducing the perturbation of specified vibrational modes with IR laser. However, in this time, the light-induced conformational changes of photoreceptor protein were not able to be analyzed by this system and we couldn' t develop the proper IR laser and installation system. Now we have continued to measure photoreceptor proteins and analyzed data.