Electrophoresis for metal ion-protein mapping

发展了一种新型的金属离子荧光探针，用于毛细管电泳-激光诱导荧光检测（CE-LIF）和聚丙烯酰胺凝胶电泳（PAGE）。几种过渡金属离子的检测限均达到几十pM。在CE-LIF中，阳离子聚合物的加入提供了金属探针络合物之间的高分辨率，具有新的分离原理。新的分离模式被命名为“离子缔合络合分离模式”。在PAGE中，成功地测定了存在于转铁蛋白和铜蓝蛋白凝胶级分中的Fe和Cu离子。

Novel fluorescent probes for detecting metal ions working in capillary electrophoresi-laser-induced fluorescence detection (CE-LIF) and polyacrylamide gel electrophoresis (PAGE) have been developed. The detection limits for several transition metal ions of tens pM were successfully obtained. In CE-LIF, the addition of cationic polymer provided high resolution among metal-probe complexes with new separation principle. The new separation mode was named as "Ion association complex separation mode." In PAGE, Fe and Cu ions which existed in the gel fractions of transferrin and ceruloplasmin were successfully determined.