Electrophoresis for metal ion-protein mapping

开发了用于毛细管电泳检测金属离子的新型荧光探针——激光诱导荧光检测（CE-LIF）和聚丙烯酰胺凝胶电泳（PAGE）。成功地获得了数十pM的几种过渡金属离子的检出限。在CE-LIF中，阳离子聚合物的加入以新的分离原理为金属探针配合物提供了高分辨率。新的分离模式被命名为“离子缔合络合物分离模式”。PAGE法成功地测定了转铁蛋白和铜蓝蛋白凝胶组分中的Fe和Cu离子。

Novel fluorescent probes for detecting metal ions working in capillary electrophoresi-laser-induced fluorescence detection (CE-LIF) and polyacrylamide gel electrophoresis (PAGE) have been developed. The detection limits for several transition metal ions of tens pM were successfully obtained. In CE-LIF, the addition of cationic polymer provided high resolution among metal-probe complexes with new separation principle. The new separation mode was named as "Ion association complex separation mode." In PAGE, Fe and Cu ions which existed in the gel fractions of transferrin and ceruloplasmin were successfully determined.