Dynamics of structural formation of osteoblastic cells responding to change in its peripheral mechanical environment

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In this study, we investigated the mechanism to sense changes in peripheral mechanical environment and effects of mechanical factors on structural changes of cytoskeleton in osteoblastic cells (MC3T3-E1) which plays an important role for mechanical adaptation of bone. We observed the reorganization process of actin stress fibers in osteoblastic cells under controlled focal adhesion complex. The morphological characteristics of the actin cytoskeleton on the substrate with/without micropatterning were observed for the fixed cells, and we confirmed that the controlled adhesion induced preferred orientation of the cells and fibers. Additionally, we found that the micropatterned substrate enhanced formation of actin stress fibers.These results suggest that density of actin-bundling and internal tensile forces in actin filaments and distribution of focal adhesion complex in cytoskeleton are one of the important factors to sense changes in peripheral mechanical environment in osteoblastic cell.