Breeding of pentose-fermenting Saccharomyces cerevisiae by metabolic engineering

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To efficiently ferment xylose derived from lignocellulotic biomass by Saccharomyces cerevisiae, I focused on extracellular pentose-uptake system of Pichia stipitis, and sugar-sensing pathway of S. cerevisiae. The identified xylose transporter gene(s) from P. stipitis and/or hexose-sensor gene from S. cerevisiae were introduced into a novel screened industrial strain(s) of S. cerevisiae, and effects on xylose-fermentation were analyzed. Furthermore, bioethanol production from water-plant was largely carried out using the genetic-engineered yeast which was constructed.