High-throughput ligand screening of nuclear receptor by using gold nanoparticle modified with co-activator peptide

点击翻译按钮获取中文摘要

We have developed functional gold nanoparticles (SRC1-GNP) for high through-put ligand screening of nuclear receptor. The SRC1-GNP was constructed to introduce the SRC-1 peptide on GNP. The SRC-1 peptide is part of co-activator, which is bound to the nuclear receptor activated by agonist. The SRC1-GNP can analyze agonist activity of nuclear receptor ligand to detect the visible spectra change of the solution. This colorimetric sensor will be specific, responsive, rapid and low-cost method for identification of lead-substances in the drug discovery.