Development of the compositive improvement technology of metabolic and sugar transport abilities for efficient bioethanol production

开发高效生产生物乙醇的代谢和糖运输能力综合改进技术

• 批准号: 21760639
• 负责人: TANINO Takanori
• 金额: $ 2.91万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21760639/
• 关键词: 再生可能エネルギー; 二酸化炭素削減; バイオマス; 発酵; 微生物; バイオエタノール; キシロースイソメラーゼ; トランスポーター; 再生可能円ルギー; 二酸化炭素排出消減; 二酸化炭素排出削減

An introduction method of the xylose isomerase pathway into the yeast by the genome integration of xylose isomerase encoding gene was constructed. Ethanol fermentation abilities from glucose and xylose of both only xylose isomerase pathway introduced yeast and xylitol, by-product of fermentation from xylose, pathway blocked that yeast were evaluated. And glucose and xylose consumption rates were improved by the overexpression of the transporter that intermediate uptake of the sugars into the yeast.

构建了通过木糖异构酶编码基因的基因组整合将木糖异构酶途径引入酵母的方法。评估了仅引入酵母和木糖发酵副产物木糖异构酶途径的葡萄糖和木糖的乙醇发酵能力，途径阻断了酵母。转运蛋白的过度表达可提高酵母对糖的吸收，从而提高了葡萄糖和木糖的消耗率。

项目成果

Improvement of the metabolic pathway network of the yeast for the efficient ethanol production from mixed sugar.

改善酵母的代谢途径网络，以从混合糖中高效生产乙醇。

• 发表时间: 2010
• 作者: T. Ohkubo;Y. Ishii and T. Kamiya;Tanino T.
• 通讯作者: Tanino T.

Construction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylitol on fermentation

将木糖异构酶基因整合到基因组中构建木糖代谢酿酒酵母并研究木糖醇对发酵的影响

• 发表时间: 2010
• 作者: T.Yanagida;Y.Fujimoto;Y.Ohgi;Y.Yokota;A.Yoshikawa;H.Kagi;K.Sugiyama;K.Kamada;谷野孝徳
• 通讯作者: 谷野孝徳

Improvement of the metabolic pathway network of the yeast for the efficient ethanol production from mixed sugar

改善酵母的代谢途径网络，以利用混合糖高效生产乙醇

• 发表时间: 2010
• 作者: T.Yanagida;A.Yoshikawa;Y.Yokota;K.Kamada;Y.Usuki;S.Yamamoto;M.Miyake;M.Baba;K.Sasaki;M.Ito;大久保猛;谷野孝徳
• 通讯作者: 谷野孝徳

Construction of a xylose-metabolic Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylitol on fermentation.

通过将木糖异构酶基因整合到基因组中构建木糖代谢酿酒酵母并研究木糖醇对发酵的影响。

• 发表时间: 2010
• 作者: T.Yanagida;Y.Fujimoto;Y.Ohgi;Y.Yokota;A.Yoshikawa;H.Kagi;K.Sugiyama;K.Kamada;谷野孝徳;Takeru Ohkubo;Tanino T.
• 通讯作者: Tanino T.

Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation

• DOI: 10.1007/s00253-010-2870-2
• 发表时间: 2010-11-01
• 期刊: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
• 影响因子: 5
• 作者: Tanino, Takanori;Hotta, Atsushi;Kondo, Akihiko
• 通讯作者: Kondo, Akihiko