Elucidation of ICE1-dependent cold signaling network

为了鉴定作为冷信号调节因子的ICE1的反式激活系统，我们引入了定点突变。丝氨酸403对丙氨酸的替代抑制了ICE1的降解，从而稳定了ICE1(S403A)。这种稳定导致了耐寒性的提高。为了了解ICE1的调控机制，我们通过酵母双杂交筛选的方法分离了ICE1的相互作用因子。MYC型转录因子、激酶和钙调素样蛋白被鉴定为ICE1相互作用因子。此外，这些结果表明，类钙调素蛋白参与了冷信号和耐冷性的调节。

To identify the transactivation system of ICE1, which is a regulator of cold signaling, site-directed mutagenesis was introduced. Substitution of serine 403 to alanine inhibited degradation of ICE1, consequently, ICE1(S403A) was stabilized. This stabilization leads to improving cold tolerance. And to understand the regulatory mechanisms of ICE1, interactors of ICE1 were isolated by yeast 2-hybrid screening. MYC-type transcription factors, kinases, and calmodulin-like proteins were identified as ICE1-interactors. Furthermore, the results suggested that calmodulin-like proteins are involved in regulation of cold signaling and cold tolerance.