Structural basis of polarity protein LGN in complex with its partner molecules

LGN与伴侣分子Insc结合的复合物在调节细胞极性方面起着至关重要的作用。为了从LGN-Insc的三级结构来阐明其相互作用，我们利用x射线晶体学对LGN-Insc配合物进行了结构研究。确定了LGN-Insc相互作用的区域作为适合结构研究的结构，然后我们建立了络合物的制备。我们对LGN-Insc配合物进行了结晶试验，优化了结晶条件，得到了适合x射线衍射的晶体。收集了LGN-Insc配合物晶体的x射线衍射数据，分辨率为2.8 Å。

A complex in which LGN associates with a partner molecule, Insc, plays a crucial role in regulating cell polarity. To elucidate the interaction between LGN and Insc based on its tertiary structure, we have been carried out structural studies of LGN-Insc compelx using by X-ray crystallography. Regions of LGN-Insc interaction as a construct suitable for structural studies were identified and then we established the preparation of the complex. We performed crystallization trials on the LGN-Insc complex and obtained crystals suitable for X-ray diffraction after optimization of crystallization condition. X-ray diffraction data were collected to a resolution of 2.8 Å for crystals of the LGN-Insc complex.