Structural basis of polarity protein LGN in complex with its partner molecules

LGN与伴侣分子iNSC结合的复合体在调节细胞极性方面起着至关重要的作用。为了从LGN-iNSC的三级结构上阐明LGN与iNSC之间的相互作用，我们用X射线结晶学方法对LGN-iNSC复合体的结构进行了研究。确定了适合于结构研究的LGN-iNSC相互作用区域，并建立了复合体的制备方法。我们对LGN-iNSC络合物进行了晶化实验，优化了晶化条件，得到了适合于X射线衍射的晶体。收集了晶体的X-射线衍射数据，分辨率为2.8Å。

A complex in which LGN associates with a partner molecule, Insc, plays a crucial role in regulating cell polarity. To elucidate the interaction between LGN and Insc based on its tertiary structure, we have been carried out structural studies of LGN-Insc compelx using by X-ray crystallography. Regions of LGN-Insc interaction as a construct suitable for structural studies were identified and then we established the preparation of the complex. We performed crystallization trials on the LGN-Insc complex and obtained crystals suitable for X-ray diffraction after optimization of crystallization condition. X-ray diffraction data were collected to a resolution of 2.8 Å for crystals of the LGN-Insc complex.