Dynamic behavior analysis of membrane molecules in glycolipid enriched membrane microdomain

膜横向异质性被认为是生物膜功能的一个要求，“筏/微区”的概念赋予了这个概念的特异性。在这项研究中，通过对荧光恢复曲线的拟合分析和对单个荧光点的痕量分析来测量IR的横向扩散，从而提供扩散系数（D）。它向我们展示了在膜环境改变时，例如胆固醇抑制刺激前后，膜分子扩散的速度有多快。我们将利用这些技术在另一种测定条件下进行膜受体的侧向扩散分析，例如鞘糖脂（GSL）抑制剂的处理，GSL缺陷细胞或病理样品的使用。

Membrane lateral heterogeneity is accepted as a requirement for the function of biological membranes, and the notion of "raft/microdomain" gives specificity to this concept. In this study, measurement of the lateral diffusion of IR was performed by fitting analysis to fluorescence recovery curves and trace analysis to individual fluorescent spots, which provided diffusion coefficient (D). It shows us how fast membrane molecules are diffusing at the change of membrane environment such as before and after stimulation by cholesterol depression. We will utilize these techniques for the lateral diffusion analysis of membrane receptor in another assay conditions, such as treatment of glycosphingolipid (GSL) inhibitor, use of GSL deficient cells or of pathologic samples.