analysis of lysophospholipid signaling in zebrafish

本研究的目的是阐明斑马鱼溶血磷脂信号转导的分子机制。我们首先利用Gal4-UAS系统和转座子(Tol2)对几个载体进行了修饰，以便于获得转基因株系。利用这些载体，我们成功地建立了几个转基因株系。这些都是有用的工具，以阐明溶血磷脂信号功能的时间和位置。我们成功地发现了与某些器官发生有关的几个基因。斑马鱼被认为是具有溶血磷脂信号相关基因的最低等模式生物。我们的研究表明，玉米鱼是阐明体内溶血磷脂信号的有用模式生物。

The aim of this study is clarifying the molecular machinery of lysophospholipid signaling using zebrafish. We first modified several vectors using gal4-UAS system and transposon (tol2) to facilitate making transgenic lines. Using this vectors, we succeeded in establishing several transgenic lines. These are useful tools to elucidate the timing and the place in which lysophospholipid signaling functions. We succeeded in discovering several genes involved in some organogenesis. Zebrafish is considered as the lowest model organisms which possesses lysophospholipid signaling related genes. Our study shows that zebafish as a useful model organisms to elucidate the lysophospholipid signaling in vivo.