Physical measurement of cell membrane by using novel optical microscopy

为了揭示丝状伪足突起的机制，我们开发了一种新型的显微镜，可以实时、高对比度地检测像CEKK膜这样的纳米结构。不幸的是，这一发展在两年内没有实现，然而，在拨款之后完成。由于目前的发展存在上述问题，我们开发了肌球蛋白-X的生物测定系统来定量肌球蛋白-X的动力学，肌球蛋白-X是丝状足突的罪魁祸首(生物化学杂志，285,19605)。此外，我们发展了超分辨率显微镜，以90 nm和80ms的时空分辨率观察细胞膜(BiPhys.J.，99，L50-52)。

In order to reveal mechanism of filopodia protrusion, we have developed a novel microscopy to detect nano structures like cekk membrane in real time with high contrast. Unfortunately, this development was not achieved for 2 year, however, done after the grant. Since the present developments have some trouble as above mentioned, we developed biological assay system to quantify the dynamics of myosin-X, which is responsible for filopodia protrusion (J Biol Chem., 285, 19605). Furthermore, we developed superresolution microscopy to observe the cell membrane with 90 nm and 80 ms spatial-temporal resolutions (Biophys.J., 99, L50-52).