Analysis of hierarchical structure of proteins network

在信号转导和胞内囊泡运输方面，由多个功能结构域组成的多结构域蛋白在生物学功能中起着核心作用。在这项工作中，阐明涉及多结构域蛋白质的分级调节的分子机制，结构域重排的多结构域蛋白质启动刺激每个结构域，并与结构域重排的分子间和分子间的调节进行了检查，通过使用X-射线散射和动态光散射。研究结果表明，GGA中参与囊泡运输和Ppr的多结构域蛋白在其功能表达过程中表现出分子间的解离和结合，而结构域重排则可能调控涉及多结构域蛋白的蛋白集合体的分子事件。

Concerning signal transduction and intracellular vesicular traffic, multi-domain proteins comprised of several functional domains act a central role in the biological functions. In this work, to elucidate the molecular mechanism of the hierarchical regulation involving multi-domain proteins, domain rearrangement of multi-domain proteins initiated by stimulating each domain, and intermolecular and intermolecular regulation associated with the domain rearrangement were examined by using x-ray scattering and dynamic light scattering. In the results, the multi-domain proteins of GGA involved in vesicular traffic and Ppr in a two component regulatory system exhibit intermolecular dissociation and association of their domains during their function expression, and the domain rearrangements are expected to regulate molecular events of protein ensembles involving the multi-domain proteins.