Functional analysis of actin-binding protein Tropomyosin4 in the spindle formation and orientation

哺乳动物的卵母细胞是高度极化的，因为不对称的纺锤体迁移到卵母细胞皮层确保了在两次减数分裂中小的极体的挤出。为了阐明维持这种极性的机制，我分析了肌动蛋白丝相关蛋白原肌球蛋白4（Tm 4）的功能。Tm 4-siRNA的缺失导致卵母细胞中纺锤体的极性和极性扩散的异常纺锤体。这些数据表明Tm 4在减数分裂过程中的纺锤体方向和染色体迁移中起着重要作用。

The mammalian oocyte is highly polarized because asymmetrical spindle migration to the oocyte cortex ensures extrusion of small polar bodies in the two meiotic divisions. To elucidate the mechanism of maintain this polarity, I analyzed the function of an actin-filament associated protein tropomyosin 4(Tm4). Tm4-depletion by siRNA causes abnormal spindles with diffused poles and polarity of spindles in oocyte. These data indicate that Tm4 plays important roles in spindle orientation and chromosome migration during meiosis.