The role of sumoylation in retinal progenitor proliferation

苏酰化在视网膜祖细胞增殖中的作用

• 批准号: 21770246
• 负责人: TERADA Koji
• 金额: $ 2.91万
• 依托单位: Osaka Bioscience Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770246/
• 关键词: Ubc9; sumo; Xenopus; retina; cell cycle; progenitor; 網膜; ubc9; hmgb3; アフリカツメガエル; 細胞増殖

Precisely controlled progenitor proliferation is essential for normal development. However, molecular mechanisms, which control the correct timing of cell cycle withdrawal during development, have been poorly understood. We show here that ubc9, a sumo-conjugating enzyme, controls the cell cycle exit of retinal progenitors. We found that ubc9 is highly expressed in retinal progenitors and stem cells in Xenopus embryos. Ubc9 physically and functionally associates with Xenopus hmgb3, which is required for retinal cell proliferation, and prolonged expression of ubc9 and hmgb3 results in suppression of the cell cycle exit of retinal progenitors in a sumoylation-dependent manner. Overexpression of ubc9 and hmgb3 decreased expression of the cell-cycle inhibitor p27^<xic1>. Furthermore, progenitor proliferation is regulated, at least in part, by sumoylation of transcription factor Sp1. These results suggest a significant role of sumoylation for cell cycle regulation in retinal progenitors

精确控制祖细胞增殖对正常发育至关重要。然而，在发育过程中控制细胞周期退出的正确时间的分子机制却知之甚少。我们在这里展示了ubc9，一种相合结合酶，控制视网膜祖细胞的细胞周期退出。我们发现ubc9在爪蟾胚胎的视网膜祖细胞和干细胞中高度表达。Ubc9在物理和功能上与爪蟾hmgb3相关，后者是视网膜细胞增殖所必需的，延长Ubc9和hmgb3的表达导致视网膜祖细胞周期退出以一种依赖于同化的方式受到抑制。过表达ubc9和hmgb3可降低细胞周期抑制剂p27^<xic1>的表达。此外，祖细胞增殖至少在一定程度上受转录因子Sp1的sumo化调节。这些结果提示sumoylation在视网膜祖细胞的细胞周期调节中起重要作用

项目成果

SMOC1 Is Essential for Ocular and Limb Development in Humans and Mice

• DOI: 10.1016/j.ajhg.2010.11.012
• 发表时间: 2011-01-07
• 期刊: AMERICAN JOURNAL OF HUMAN GENETICS
• 影响因子: 9.8
• 作者: Okada, Ippei;Hamanoue, Haruka;Saitsu, Hirotomo
• 通讯作者: Saitsu, Hirotomo

Sumoylation activity of Ubc9 is essential for retinal stem/progenitor cell proliferation

Ubc9 的苏酰化活性对于视网膜干/祖细胞增殖至关重要

• 发表时间: 2009
• 作者: 池谷真;他;寺田晃士
• 通讯作者: 寺田晃士

Sumoylation activity of Ubc9, a SUMO-conjugating enzyme, is essential for retinal stem/prosenitor cell proliferation

Ubc9（一种 SUMO 结合酶）的苏酰化活性对于视网膜干/祖细胞增殖至关重要

• 发表时间: 2009
• 作者: Mikihito Shibata;Hiromi Nakao;Hiroshi Kiyonari;Takaya Abe;Shinichi Aizawa;寺田晃士
• 通讯作者: 寺田晃士

SUMO結合酵素Ubc9は網膜幹細胞・未分化前駆細胞の増殖を制御する

SUMO结合酶Ubc9调节视网膜干细胞和未分化祖细胞的增殖

• 发表时间: 2009
• 作者: 池谷真;他;寺田晃士
• 通讯作者: 寺田晃士

Takahisa Furukawa Sumoylation activity of Ubc9 is essential for retinal stem/progenitor cell proliferation

Takahisa Furukawa Ubc9 的 Sumoylation 活性对于视网膜干/祖细胞增殖至关重要

• 发表时间: 2009
• 作者: Mikihito Shibata;Hiromi Nakao;Hiroshi Kiyonari;Takaya Abe;Shinichi Aizawa;Koji Terada
• 通讯作者: Koji Terada