Development of an efficient chromatin pull-down method from small cell numbers

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The aim of this research project is to develop a method to determine the binding sites of specific transcription factors that play important roles in specific cell lineages, such as primordial germ cells. To purify genomic DNA bound to a specific transcription factor, I developed knock-in mice that carry tags in the particular transcription factor. Furthermore, I developed a method to quantitatively amplify genomic DNA bound to a transcription factor. By these methods, it has become plausible to determine genomic sites bound to a transcription factor in a small cell number, such as 10, 000 cells.