Intensive mapping of diplospory gene locus and exploitation of candidate gene for diplospory in Chinese chive

韭菜双孢子基因座精细定位及双孢子候选基因开发

• 批准号: 21780030
• 负责人: YAMASHITA Ken-ichiro
• 金额: $ 2.83万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780030/
• 关键词: アポミクシス; アリウム; 単為発生性; ニラ; 複相大胞子形成性; 複相大胞子形成; 単為発生; Allium; トリソミック; DNAマーカー; ニフ

Genetic maps of diplospory(D) and parthenogenesis(P) gene loci were constructed by linkage analysis with AFLP, RAPD and ISSR markers in the heteroploid population(BC1) in Chinese chive. Degrees of diplospory and parthenogenesis were evaluated in the diploid-hyperdiploid population(BC2) produced by the cross between amphimictic diploid and apomictic hypotriploid BC1 plants. As the results, four plants with low diplospory values and one plant with relatively high parthenogenesis value were identified. By the Feulgen staining of root tip cells in the parthenogenetic plant, it was revealed that this plant was a trisomic(2n=17, 2x+1), which indicates that the P locus is located on the additional chromosome.

利用AFLP、RAPD和ISSR分子标记对韭菜异倍体群体（BC 1）进行连锁分析，构建了二倍体孢子生殖（D）和孤雌生殖（P）基因位点的遗传图谱。在由两性融合二倍体和无融合生殖亚三倍体BC 1植物之间的杂交产生的二倍体-超二倍体群体（BC 2）中评估了倍数孢子体形成和孤雌生殖的程度。结果，鉴定出4株倍数孢子体形成值较低的植株和1株孤雌生殖值较高的植株。通过对孤雌生殖植株根尖细胞进行Feulgen染色，发现该植株为三体（2n=17，2x+1），表明P基因位于附加染色体上。

项目成果

Modes of inheritance of two apomixis components, diplospory and parthenogenesis, in Chinese chive (Allium ramosum) revealed by analysis of the segregating population generated by back-crossing between amphimictic and apomictic diploids

通过分析两性和无性生殖二倍体回交产生的分离群体，揭示了韭菜（Allium ramosum）中两种无融合生殖成分（双倍体和单性生殖）的遗传模式

• 期刊: Breeding Science
• 影响因子: 2.4
• 作者: Yamashita K.;Y. Nakazawa;K. Namai;M. Amagai;H. Tsukazaki;T. Wako;A. Kojima;山下謙一郎
• 通讯作者: 山下謙一郎

ニラ両性生殖性二倍体×アポミクシス性低三倍体による単為発生性トリソミックの作出

香葱雌雄同体二倍体 x 无融合生殖亚三倍体创建孤雌三体

• 发表时间: 2012
• 作者: 山下謙一郎;塚崎光;小島昭夫;谷口成紀;若生忠幸
• 通讯作者: 若生忠幸

ニラ異数性分離集団を用いた複相大胞子形成遺伝子座および単為発生遺伝子座のマッピング

使用细香葱非整倍体分离群体绘制二相大孢子发生位点和孤雌生殖位点

• 发表时间: 2011
• 作者: 山下謙一郎;塚崎光;若生忠幸;小島昭夫
• 通讯作者: 小島昭夫

Modes of Inheritance of two apomixis components, diplospory and parthenogenesis, in Chinese chive(Allium ramosum) revealed by analysis of the segregating population generated by back-crossing between amphimictic and apomictic diploids

通过分析两性和无性生殖二倍体回交产生的分离群体揭示韭菜（Allium ramosum）两种无融合生殖成分（双倍体和单性生殖）的遗传模式

• 发表时间: 2012
• 期刊: Breeding Science
• 影响因子: 2.4
• 作者: Yamashita K.;Y. Nakazawa;K. Namai;M. Amagai;H. Tsukazaki;T. Wako;A. Kojima
• 通讯作者: A. Kojima

Modes of Inheritance of Two Apomixis Components, Diplospory and Parthenogenesis, in Chinese chive(Allium ramosum)

韭菜双性生殖和单性生殖两种无融合生殖成分的遗传模式

• 发表时间: 2012
• 作者: Yamashita;K.;Y. Nakazawa;K. Namai;M. Amagai;H. Tsukazaki;T. Wako;A. Kojima
• 通讯作者: A. Kojima