Intensive mapping of diplospory gene locus and exploitation of candidate gene for diplospory in Chinese chive

利用AFLP、RAPD和ISSR分子标记，构建了中国香葱异倍体群体(Bc1)双孢子(D)和孤雌生殖(P)基因座的遗传图谱。对二倍体-超二倍体群体(BC2)的双孢子和孤雌生殖程度进行了研究。结果鉴定出4株复孢子值较低的植株和1株较高的孤雌生殖植株。对孤雌生殖植株的根尖细胞进行Feulgen染色，结果表明该植株为三体(2n=17，2x1)，表明P基因座位于附加染色体上。

Genetic maps of diplospory(D) and parthenogenesis(P) gene loci were constructed by linkage analysis with AFLP, RAPD and ISSR markers in the heteroploid population(BC1) in Chinese chive. Degrees of diplospory and parthenogenesis were evaluated in the diploid-hyperdiploid population(BC2) produced by the cross between amphimictic diploid and apomictic hypotriploid BC1 plants. As the results, four plants with low diplospory values and one plant with relatively high parthenogenesis value were identified. By the Feulgen staining of root tip cells in the parthenogenetic plant, it was revealed that this plant was a trisomic(2n=17, 2x+1), which indicates that the P locus is located on the additional chromosome.