Genetic engineering for production of copolymerized biopolyester from C1compounds

本研究旨在构建以甲醇为原料生产柔性聚羟基烷酸(PHA)共聚物的重组甲基营养细菌，并以该菌为宿主菌进行研究。结果表明，Extorquens野生型菌株积累了以C4单体为主、少量C5单元组成的PHA共聚物。成功地获得了能积累由C4、C5和C6单元组成的PHA的重组菌株。这些菌株比野生型菌株更高效地产生PHA。

This study is aimed at construction of recombinant methylotrophic bacteria to produce flexible polyhydroxyalkanoate(PHA) copolymers from methanol using Methylobacterium extorquens AM1 as a host strain. The results revealed that M. extorquens wild-type strain accumulates PHA copolymer composed of mostly C4 monomer with a small amount of C_5 unit. The recombinant strains, which can accumulate PHA composed of C_4, C_5, and C_6 unit, was successfully obtained. These strains produced PHA more efficiently than wild-type strain.