Identification of the biosynthetic genes for antifungal drugMicafungin by ATMT.

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T-DNA insertional mutagenesis by Agrobacterium tumefaciens-mediated transformation (ATMT) and whole genomic DNA sequencing analysis with next generation sequencer for FR901379 producing fungus Coleophoma empetri F-11899 derived putative biosynthetic gene cluster encoding FR9013797. However, the region of gene cluster of FR901379 was so huge and containing various coding genes such as unknown function and oxidoreductases, that it was difficult to speculate the pathway for FR901379 biosynthesis without proof of each gene disruption.