Analysis of a highly stable cholesterol oxidase from a gram-negative bacterium

胆固醇氧化酶是一种很有价值的酶，用于测定人体血液中的胆固醇。本研究从一株革兰氏阴性菌Chromobacterium sp. DS-1中发现了一种耐热、耐有机溶剂和洗涤剂的胆固醇氧化酶。结果发现，辅酶FAD参与酶的稳定性。

Cholesterol oxidase is a valuable enzyme which is used for determination of cholesterol in human blood. We previously discovered a thermal, organic solvent and detergent-tolerant cholesterol oxidase from a gram-negative bacterium Chromobacterium sp. DS-1.In this study, the mechanism of the high stability of the enzyme was studied. As the result, it was found that a coenzyme FAD is involved in the stability of the enzyme.