Organohalide respiration in the subseafloor microbial ecosystem-Investigation of RDH genes and dehalogenation activity

海洋沉积物中的卤化有机物是厌氧微生物呼吸的电子受体之一。在秘鲁附近的东南太平洋、赤道东太平洋、俄勒冈州附近的胡安德富卡海脊和日本附近的西北太平洋的沉积物中总共检测到32个可能的还原脱卤酶同源基因，这些沉积物的最大海底深度为358.6米。此外，在南开海槽弧前盆地底泥中观察到了对2，4，6-三溴苯酚、2，4，6-三碘苯酚和三氯乙烯的脱卤化活性。这些结果表明，脱盐呼吸是海底微生物生态系统中一条重要的能量产生途径。

Halogenated organic matter incorporated in marine sediments is one source of electron acceptors for anaerobic microbial respiration. A total of 32 putative reductive dehalogenase homologous genes were detected in sediments from the southeast Pacific off Peru, the eastern equatorial Pacific, the Juan de Fuca Ridge flank off Oregon, and the northwest Pacific off Japan, collected at a maximum depth of 358.6 m below the seafloor. In addition, dehalogenation activity toward 2,4,6-tribromophenol, 2,4,6-triiodophenol, and trichloroethene was observed in sediment slurry from the Nankai Trough Forearc Basin. These results suggest that dehalorespiration is an important energy-yielding pathway in the subseafloor microbial ecosystem.