Functional and structural analyses of the archaeal L-proline dehydrogenase complexes

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Two different types of ProDHs (α_4β_4 and αβγδ type) were distributed in the hyperthermophilic archaea. Functional and structural characteristics of both ProDHs were studied. Structure determination at high resolution of α_4β_4-type ProDH which the ICS was not broken, and mutational analysis of intersubunit disulfide bond in α subunit of α_4β_4-type ProDH were performed. Screening of crystallization condition of αβγδ type ProDH was also performed by preparing the ISC mutant enzymes. And the enzymatic properties of β subunits of both types of ProDHs were analyzed.