Combinatorial biosynthesis of plant triterpenoids in engineered yeasts

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Triterpenoids, with increasing evidence of their health beneficial properties, is a diverse group of secondary metabolites. Cytochrome P450 monooxygenases (P450s) play critical roles in site-specific oxidations of triterpene skeleton ; thereby create structural variation of triterpenoid aglycones. In this study, we identified several P450 enzymes involved in triterpenoid biosynthesis by using "homology-based" and "gene co-expression analysis" methods. These identified P450s were coexpressed with oxidosqualene cyclase, such as β-amyrin synthase, in engineered yeast to produce rare-natural or non-natural terpenoid products.