Characterization and function of the urea transporter genes from Porphyra yezoensis (Rhodophyta) in molecular mechanisms underlying assimilation of nitrogen sources

条斑紫菜（红藻）尿素转运蛋白基因在氮源同化分子机制中的特征和功能

• 批准号: 21780196
• 负责人: KAKINUMA Makoto
• 金额: $ 3万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780196/
• 关键词: 紅藻; スサビノリ; トランスポーター; 窒素同化; 色落ち; 栄養塩; 環境応答; 尿素

Two urea transporters (PyUT1 and PyUT2), which may be involved in nitrogen uptake and assimilation, have been identified in the rhodophyta seaweed Porphyra yezoensis. Expression of both PyUT1 and PyUT2 genes were rapidly induced under low nitrogen concentration, suggesting a possibility that the PyUT genes play an important role in adaptation to low nitrogen conditions. The expression levels of both PyUT1 and PyUT2 genes were affected by inorganic nitrogen sources and an organic nitrogen urea. When the yeast UT (DUR3) gene-mutant strain was transformed with the plasmid vector expressing PyUT1 or PyUT2 proteins, the growth complementation of these transformants was not observed. These results suggest that the PyUT genes play important roles in uptake of other nitrogen sources and/or in intracellular urea transport system.

在条斑紫菜（Porphyrayezoensis）中发现了两种尿素转运蛋白（PyUT 1和PyUT 2），它们可能参与氮的吸收和同化。PyUT 1和PyUT 2基因的表达在低氮浓度下被迅速诱导，表明PyUT基因在适应低氮条件中起重要作用。PyUT 1和PyUT 2基因的表达水平受到无机氮源和有机氮尿素的影响。当用表达PyUT 1或PyUT 2蛋白质的质粒载体转化酵母UT（DUR 3）基因突变株时，未观察到这些转化体的生长互补。这些结果表明，PyUT基因在其他氮源的吸收和/或在细胞内尿素转运系统中发挥重要作用。

项目成果

Molecular analysis of physiological responses to environmental changes in marine macroalgae and angiosperms.Forth Bilateral Seminar Italy and Japan

海洋大型藻类和被子植物对环境变化生理反应的分子分析。第四届意大利和日本双边研讨会

• 发表时间: 2010
• 作者: Kakinuma M;Morita T;Tominaga H;Inoue M;Okumura E;Coury DA;Maegawa M;Amano H.
• 通讯作者: Amano H.