Development of eastern blotting technique for high-throughput analysis of kampo medicines

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We demonstrated the high-throughput analysis for natural glycosides in kampo medicines using monoclonal antibodies (MAbs) and eastern blotting technique. Then, rapid, simple, high-sensitive and specific detection system was developed for GC and BI by eastern blotting technique using dot blot and chemiluminescent methods. This methods was useful as the high-throughput analysis for the determination of active compounds in kampo medicines as well as HPLC and ELISA systems.