Multifunctionalization of polymer surfaces for developing advanced cell culture substrate to support cell culture applications

聚合物表面多功能化，用于开发先进的细胞培养基质以支持细胞培养应用

• 批准号: 21790038
• 负责人: SASAI Yasushi
• 金额: $ 2.58万
• 依托单位: Gifu Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790038/
• 关键词: ポリスチレン; 高分子表面機能化; 表面界面物性; 原子移動ラジカル重合; プラズマ加工; バイオインターフェイス; 生体分子固定化; 細胞接着; 細胞培養; X線光電子分光法; 細胞接着性ペプチド

In order to prepare the functional substrate surfaces used in advanced cell culture applications, two methods were developed to introduce a large amount of carboxyl groups onto the chemically-inert polystyrene(PS) substrate. In one method, the biocompatible vinylmethylether-maleic acid copolymer(VEMAC) was immobilized on PS substrate through a coupling between carboxyl groups of VEMAC and hydroxyl groups on argon plasma-irradiated PS. In the other method, the well-defined poly(acrylic acid) brushes were fabricated on PS substrate by surface-initiated atom transfer radical polymerization of sodium acrylate. The surface carboxyl groups thus introduced were used for introducing a bioactive peptide interacting with cell surface. The immobilized peptide was specifically recognized by cell membrane receptors to stimulate cell adhesion and proliferation on the surface.

为了制备用于高级细胞培养应用的功能底物表面，开发了两种方法来在化学惰性聚苯乙烯（PS）底物上引入大量羧基。一种方法是将具有生物相容性的乙烯基甲基醚-马来酸共聚物（VEMAC）通过羧基与氩气等离子体辐照的乙烯基甲基醚-马来酸共聚物（VEMAC）的羟基偶联固定在PS底物上，另一种方法是通过丙烯酸钠的表面引发原子转移自由基聚合在PS底物上制备具有良好定义的聚丙烯酸刷。由此引入的表面羧基用于引入与细胞表面相互作用的生物活性肽。固定化肽被细胞膜受体特异性识别，刺激细胞在表面的粘附和增殖。

项目成果

Plasma-assistedfhnctionalization of polystyrene surface to enhance cell adhesion and prolifbration

聚苯乙烯表面的等离子体辅助功能化增强细胞粘附和增殖

• 发表时间: 2010
• 作者: 閔俊哲;矢野裕也;豊岡利正;石清;稲垣真輔;Yoshikazu Inoh;友田敬士郎;笹井泰志
• 通讯作者: 笹井泰志

Plasma Surface Modification of Polymer Substrate for Cell Adhesion Control

• DOI: 10.2494/photopolymer.23.595
• 发表时间: 2010-06
• 期刊: Journal of Photopolymer Science and Technology
• 影响因子: 0.8
• 作者: Y. Sasai;S. Kondo;Y. Yamauchi;M. Kuzuya

細胞接着制御を目的としたプラズマ技術を利用する高分子表面修飾

使用等离子体技术进行聚合物表面修饰以控制细胞粘附

• 发表时间: 2010
• 作者: 唐木美由紀、綾野絵理;他;友田敬士郎;笹井泰志
• 通讯作者: 笹井泰志

Plasma-assisted functionalization of polystyrene surface to enhance cell adhesion and proliferation

聚苯乙烯表面的等离子体辅助功能化以增强细胞粘附和增殖

• 发表时间: 2010
• 作者: Nakanishi;M.;Inoh;Y.;Kitamoto;D.;Furuno;T.;畠中俊;綾野絵理;伊納義和;友田敬士郎;笹井泰志
• 通讯作者: 笹井泰志

Immobilization of bioactive molecules onto polymer substrate functionalized by plasma techniques and its application to cell culture

等离子体技术将生物活性分子固定在功能化聚合物基质上及其在细胞培养中的应用

• 发表时间: 2009
• 期刊: Journal of photopolymer science and technology 22
• 作者: Yasushi Sasai;Shin-ichi Kondo;Yukinori Yamauchi;Masayuki Kuzuya
• 通讯作者: Masayuki Kuzuya