Multifunctionalization of polymer surfaces for developing advanced cell culture substrate to support cell culture applications

为了制备用于高级细胞培养应用的功能底物表面，开发了两种方法来在化学惰性聚苯乙烯（PS）底物上引入大量羧基。一种方法是将具有生物相容性的乙烯基甲基醚-马来酸共聚物（VEMAC）通过羧基与氩气等离子体辐照的乙烯基甲基醚-马来酸共聚物（VEMAC）的羟基偶联固定在PS底物上，另一种方法是通过丙烯酸钠的表面引发原子转移自由基聚合在PS底物上制备具有良好定义的聚丙烯酸刷。由此引入的表面羧基用于引入与细胞表面相互作用的生物活性肽。固定化肽被细胞膜受体特异性识别，刺激细胞在表面的粘附和增殖。

In order to prepare the functional substrate surfaces used in advanced cell culture applications, two methods were developed to introduce a large amount of carboxyl groups onto the chemically-inert polystyrene(PS) substrate. In one method, the biocompatible vinylmethylether-maleic acid copolymer(VEMAC) was immobilized on PS substrate through a coupling between carboxyl groups of VEMAC and hydroxyl groups on argon plasma-irradiated PS. In the other method, the well-defined poly(acrylic acid) brushes were fabricated on PS substrate by surface-initiated atom transfer radical polymerization of sodium acrylate. The surface carboxyl groups thus introduced were used for introducing a bioactive peptide interacting with cell surface. The immobilized peptide was specifically recognized by cell membrane receptors to stimulate cell adhesion and proliferation on the surface.