Multifunctionalization of polymer surfaces for developing advanced cell culture substrate to support cell culture applications

为了制备用于先进细胞培养的功能基板表面，开发了两种方法在化学惰性聚苯乙烯(PS)基板上引入大量的羧基。在一种方法中，生物相容的乙烯基甲醚-马来酸共聚物(VEMAC)通过VEMAC的羧基与经Ar等离子体辐照的PS上的羟基之间的偶联而固定在PS基片上。在另一种方法中，通过表面引发的丙烯酸钠原子转移自由基聚合，在PS基片上制备了规整的聚丙烯酸刷子。由此引入的表面羧基被用来引入与细胞表面相互作用的生物活性多肽。固定化的多肽被细胞膜受体特异性识别，刺激细胞在表面的黏附和增殖。

In order to prepare the functional substrate surfaces used in advanced cell culture applications, two methods were developed to introduce a large amount of carboxyl groups onto the chemically-inert polystyrene(PS) substrate. In one method, the biocompatible vinylmethylether-maleic acid copolymer(VEMAC) was immobilized on PS substrate through a coupling between carboxyl groups of VEMAC and hydroxyl groups on argon plasma-irradiated PS. In the other method, the well-defined poly(acrylic acid) brushes were fabricated on PS substrate by surface-initiated atom transfer radical polymerization of sodium acrylate. The surface carboxyl groups thus introduced were used for introducing a bioactive peptide interacting with cell surface. The immobilized peptide was specifically recognized by cell membrane receptors to stimulate cell adhesion and proliferation on the surface.