Functional analysis of microtubule regulating factors in filial imprinting

孝道印记微管调节因子的功能分析

• 批准号: 21790094
• 负责人: YAMAGUCHI Shinji
• 金额: $ 2.75万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790094/
• 关键词: 分子生物学; 生化学; 行動神経科学; 刻印付け; 記憶; 行動; 細胞骨格; MAP2

Birds have a diverse behavioral repertoire and are thus ideal models for experimental analyses of neural and behavioral plasticity. The domestic chick has been intensively studied as a model for filial imprinting. To elucidate the molecular processes underlying the neural mechanism of filial imprinting in newly-hatched chicks more extensively, we previously conducted a cDNA microarray and the quantitative RT-PCR study. One of the genes was microtubule-associated protein 2 (MAP2), which is involved in cytoskeletal organization. The levels of MAP2 transcripts and proteins were increased in the mesopallium and the hippocampus after imprinting training. Here, we used in vivo gene-transfer technique we have originally developed and showed that transfected microRNA vectors preferentially silence exogenous DNA expression in neuronal cells. Using this system, the up-regulation of MAP2 accompanying filial imprinting was suppressed in vivo, which impaired the filial imprinting in chicks. Our results suggest that the regulation of MAP2 expression is required for filial imprinting.

鸟类具有多样化的行为能力，因此是神经和行为可塑性实验分析的理想模型。家养小鸡作为孝顺印记的模型已被深入研究。为了更广泛地阐明新孵出的雏鸡孝道印记神经机制背后的分子过程，我们之前进行了 cDNA 微阵列和定量 RT-PCR 研究。其中一个基因是微管相关蛋白 2 (MAP2)，它参与细胞骨架的组织。印记训练后，中皮层和海马体中 MAP2 转录本和蛋白质的水平有所增加。在这里，我们使用了我们最初开发的体内基因转移技术，并表明转染的 microRNA 载体优先沉默神经元细胞中的外源 DNA 表达。使用该系统，体内伴随着子女印记的 MAP2 上调被抑制，从而损害了雏鸡的子女印记。我们的结果表明 MAP2 表达的调节是子代印记所必需的。

项目成果

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Bioluminescence imaging of c-fos gene expression accompanying filial imprinrting in the newly hatched chick brain.

新孵出的小鸡大脑中 c-fos 基因表达伴随子女印记的生物发光成像。

• 发表时间: 2010
• 期刊: Neuroscience Research (in press)
• 作者: Yamaguchi;et al.
• 通讯作者: et al.

ニワトリヒナ刻印付けに関わる新たな大脳領域の同定

鉴定出参与雏鸡印记的新大脑区域

• 发表时间: 2009
• 作者: 上四元純;多胡めぐみ;園田よし子;笠原忠;北島孝明
• 通讯作者: 北島孝明

鳥類刻印付けにおける神経骨格制御因子Microtubule A ssociated Protein2 (MAP2)の解析

禽类印记中神经骨骼调节微管 A 相关蛋白 2 (MAP2) 的分析

• 发表时间: 2009
• 作者: 齋藤僚;片桐幸子
• 通讯作者: 片桐幸子

Suppression of the ecdysteroid-triggered growth arrest by a novel Drosophila membrane steroid binding protein.

通过新型果蝇膜类固醇结合蛋白抑制蜕皮类固醇触发的生长停滞。

• 发表时间: 2009
• 期刊: FEBS Lett. 583
• 作者: Fujii-Taira;I.;Yamaguchi;S.;Iijima;R.;Natori;S.;Homma;K.J.
• 通讯作者: K.J.