Search for anti-diabetic constituents from medicinal foods-through the enhancement of glucose metabolism in hepatocytes-

为了研究药用食品中的抗糖尿病成分，我们进行了以下筛选试验：(1)抑制高糖诱导的甘油三酯(TG)在HepG2中的积累；(2)对高糖预处理的HepG2中甘油三酯代谢的影响；(3)对HepG2中葡萄糖消耗的影响。结果表明，酰化的黄酮苷、甜菊糖和菊花苷可降低高糖预处理的HepG2中的甘油三酯含量，增加葡萄糖的消耗。此外，给予螺旋菊苷可改善ddy小鼠的糖耐量。此外，我们还发现海藻内酯类香豆素可改善胰岛素诱导的HepG2细胞的葡萄糖消耗，而新胡芦巴苷II可降低高糖预处理的HepG2细胞中的甘油三酯含量。

In order to investigate anti-diabetic constituents from medicinal foods, we examined following screening tests : (1) inhibitory effects on high glucose-induced triglyceride (TG) accumulation in HepG2 (2) effects on TG metabolism in high glucose pre-treated HepG2 (3) effects on glucose consumption in HepG2. As the results, acylated flavonol glycosides, tiliroside and helichrysoside, reduced TG contents in high glucose pre-treated HepG2 and enhanced glucose consumption in HepG2. In addition, administration of helicrysoside improved glucose tolerant in ddY mice. Furthermore, we found that isopteryxin, a khellactone-type coumarine, improved insulin-induced glucose consumption in HepG2 cells, and that neosedumoside II, a megastigmane glycoside, reduced TG contents in high glucose pre-treated HepG2.