The role of the excitatory action of GABA in the mouse estrous cycle.

我研究了GABA反应的兴奋作用对GnRH神经元生理学的功能影响。利用四环素调控的基因表达系统获得转基因小鼠。在本实验小鼠中，KCC2在特定时间点可逆地限制在GnRH神经元中过度表达。这只转基因小鼠不能显示怀孕。这些结果提示，GnRH神经元中GABA的兴奋作用在动情周期的调节中具有重要作用。

I investigated the functional consequences of excitatory action of GABA responses on GnRH neuron physiology. A transgenic mouse was generated using tetracycline controlled gene expression system. In this mouse, KCC2 was overexpressed restricted in GnRH neurons reversibly in specific time point. This transgenic mouse could not show the pregnancy. These results suggested that the excitatory action of GABA in GnRH neurons has the important role in the regulation of the estrous cycle.