Expression and function of RECK in human osteoarthritic subchondral bone tissue

应用免疫组织化学方法检测RECK在人骨关节炎(OA)软骨下骨组织中的表达。我们发现软骨下成骨细胞，尤其是胞质饱满的立方体成骨细胞，表达RECK蛋白。体外培养的人骨关节炎软骨下骨成骨细胞和小鼠成骨样细胞系MC3T3-E1均表达RECK。在体外成骨细胞分化过程中，RECK的表达上调。表达水平还与培养细胞密度有关。我们建立了RECK下调这些细胞的方法

Expression of RECK in human osteoarthritic (OA) subchondral bone tissue was examined by immunohistochemistry. We found that the subchondral bone osteoblasts, especially those with plump cuboidal cytoplasm, express RECK protein. We showed that cultured osteoblastic cells isolated from human OA subchondral bone and the murine osteoblast-like cell line MC3T3-E1 express RECK. The expression of RECK in these cells was up-regulated during osteoblastic differentiation in vitro. The expression level was also related to cell density in culture. We established the method of RECK down-regulation in these cells