Analysis on the roles of DOT1L histone methyltransferase in spermatogenesis

尽管DOT1L在生物化学和分子生物学中的功能已被充分研究，但DOT1L H3K79甲基转移酶与哺乳动物生理现象的相互作用仍有待阐明。在本研究中，我们通过分析雄性生殖系特异性mDot1l基因敲除小鼠和其他方法证明mDot1l是精子发生所必需的。本研究为揭示DOT1L的新功能提供了基础证据，同时也阐明了组蛋白甲基转移酶在精子发生中的作用。

The interaction between DOT1L H3K79 methyltransferase and physiological phenomenon in mammals remains to be clarified, though the functions of DOT1L in biochemistry and molecular biology have been well investigated. In this study we demonstrated mDot1l is essential for spermatogenesis by analysis of male germline specific mDot1l knockout mice and other methods. From this study we obtained a basic evidence to lead us to reveal a new function of DOT1L as well as clarified a role of a histone methyltransferase in spermatogenesis.