Analyses of functions of deregulated miRNAs in renal carcinoma

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It has recently been reported that microRNAs (miRNAs), non-protein-coding small RNAs comprising about 22 nucleotides, are aberrantly expressed in certain carcinomas and play oncogenic or tumor-suppressive roles in carcinoma cells. The aim of this project is to clarify the functions of the miRNAs aberrantly expressed in clear cell renal cell carcinoma (CCC), and to identify miRNAs associated with carcinogenesis and/or cancer progression.Three miRNAs are found deregulated in CCC, but the mechanism and biological consequences of their deregulation are poorly understood. MiR-200c and miR-141 are significantly downregulated in CCC. Ectopic expression of these miRNAs induced growth suppression of renal carcinoma cell lines. Ectopic expression of miR-210, which is upregulated in CCC, caused accumulation at G2/M phase of the cell cycle associated with multipolar spindle, suggesting that miR-210 overexpression may trigger an event that hinders normal cell division.