Basic researches for developing new liver regeneration therapy using cultured autologous bone marrow derived cells in decompensated liver cirrhosis patients with hepatocellular carcinomas.

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Objectives : To evaluate the effects of autologous bone marrow cell infusion (ABMi) on the mechanisms of hepatocarcinogenesis, we performed frequent BMi in hepatocarcinogenic mice with liver cirrhosis. Methods : A mouse model of hepatocarcinogenesis with liver cirrhosis (DEN/GFP-CCl_4 model) was developed. In the BMi group, GFP-positive bone marrow cells from syngeneic mice were infused via the tail vein in DEN/GFP-CCl_4 model mice. Kinetics of hepatocarcinogenesis were histologically evaluated. Results : In the BMi group, foci and tumor showed significantly lower incidence and smaller number, while size was almost equal. Moreover, hepatic 8-OHdG levels were significantly lower. Conclusions : These results suggested that frequent ABMi might contribute the suppressed tumor-initiation and no tumor-promotion during stages of hepatocarcinogenesis.