Exploration of novel therapeutic targets of human pancreatic cancers by miRNA expressing vectors and DNA microarrays

胰腺癌是一种典型的难治性实体癌，为了探索胰腺癌治疗的新靶点，我们利用microRNA表达病毒库和定制的微阵列技术建立了一种功能性筛选方法。使用这种功能性筛选试验，我们探索了控制胰腺癌细胞增殖的microRNA，并鉴定了五种显著抑制细胞增殖的microRNA。这些microRNA及其靶点似乎是胰腺癌有希望的新的治疗靶点。

To explore new therapeutic targets of pancreatic cancer, which is a representative hard-to-cure solid caner, we developed a functional screening assay using microRNA expressing virus library and custom-made microarrays. Using this functional screening assay, we explored microRNAs that control proliferation of pancreatic cancer cells and identified five microRNA that remarkably repress cell proliferation. These microRNAs and their targets appear to be promising novel therapeutic targets of pancreatic cancers.