Understanding of significance of angiogenesis/hypoxia-inducible transcription factors and development of novel therapy for rheumatoid arthritis

缺氧和铁螯合剂或CoCl 2依赖性HIF-1α蛋白稳定在小鼠源性细胞中以时间依赖性方式得到证实。继续建立表达HIF-1α特异性siRNA的构建体。实时荧光定量PCR检测HIF-1靶基因。利用腺病毒载体表达HIF-1α的表达系统正在构建中，该系统可用于siRNA敲除HIF-1 α，或表达组成型活性HIF-1α和显性失活HIF-1α。

Hypoxia and iron chelator or CoCl2 dependent HIF-1α protein stabilization in time dependent manner was confirmed in mouse-derived cells. Establishment of a constuct expressing HIF-1α specific siRNA is continued. Quantitative analyses of HIF-1 target genes were carried out by real-time PCR. Expression system by Adenovirus vector for siRNA knocking down HIF-1α, or expressing constitutive active HIF-1α, and dominant negative HIF-1α is under construction.