A study of mobile element inserted into dystrophin gene

我们鉴定了一个大约330-bp的插入到dystrophin基因中的序列。该插入序列具有约110-bp的T延伸，并且剩余的212-bp的互补序列与人类11号染色体上的基因组序列完全匹配。我们的研究结果表明，11号染色体上的源区域被插入到dystrophin基因逆转录后。对转录本的进一步分析显示，11号染色体上的整个452-bp区域被活跃地转录。这个452 bp的序列是从一个没有基因被定位的区域转录的，没有明显的蛋白质编码能力。因此，该转录本目前被认为是一种新的非编码基因。

We identified an approximately 330-bp sequence inserted into dystrophin gene. This inserted sequence had an approximately 110-bp stretch of T, and complementary sequence of the remaining 212-bp perfectly matched with genomic sequence on the human chromosome 11. Our finding suggested that the source region on chromosome 11 was inserted into dystrophin gene after reverse transcription. Further analysis of the transcript revealed that entire 452-bp region on chromosome 11 was actively transcribed. This 452-bp sequence was transcript from a region where no gene had been mapped and had no appreciable protein coding ability. Thus this transcript is currently considered a novel non-coding gene.