Relation of ion channel function of transporter and ABCA3 in alveolar epithelial cells

姜黄素对G551D-CFTR全细胞和单通道电流的增加作用小于染料木素在其最大有效浓度下的作用。然而，姜黄素进一步增加了已经被染料木素最大程度增强的G551D-CFTR的通道活性，最高可达WT-CFTR水平的^-50%。姜黄素和金雀异黄素之间的相加效应不仅支持CFTR增强子的作用涉及多个机制的假说，而且还表明CFTR和ABCA3之间存在药学关系，因为CFTR和ABCA3是ABC蛋白家族成员，并以NBD为驱动引擎。由于原代培养的小鼠肺泡II型上皮细胞能够稳定培养，我们用相干反斯托克斯拉曼散射(CARS)显微镜观察了II型肺泡上皮细胞中的板层小体。可观察到大量表达ABCA3的板层小体，向板层小体堆积脂质，并向细胞外胞吐。

Curcumin increased G551D-CFTR whole-cell and single-channel currents less than genistein did at their maximally effective concentrations. However, curcumin further increased the channel activity of G551D-CFTR that had been already maximally potentiated by genistein, up to^-50% of the WT-CFTR level. The additive effects between curcumin and genistein not only support the hypothesis that multiple mechanisms are involved in the action of CFTR potentiators, but also suggests pharmaceutical relation between CFTR and ABCA3, because CFTR and ABCA3 are ABC protein family member and having NBD as a driving engine. Since primary culture of type II alveolar epithelial cells from the mouse, was able to made stable, we observed lamellar bodies in type II alveolar epithelial cells, using Coherent Anti-Stokes Raman Scattering(CARS) microscope. We could observe a lot of lamellar bodies expressing ABCA3, packing lipids to lamellar body and exocytosis to the outside of these cells.