Relation of ion channel function of transporter and ABCA3 in alveolar epithelial cells

肺泡上皮细胞转运蛋白与ABCA3离子通道功能的关系

• 批准号: 21791041
• 负责人: MATSUZAKI Yohei
• 金额: $ 2.66万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791041/
• 关键词: 新生児医学; ABCA3; 肺胞上皮; イオンチャネル; パッチクランプ; サーファクタント; ハッチクランプ

Curcumin increased G551D-CFTR whole-cell and single-channel currents less than genistein did at their maximally effective concentrations. However, curcumin further increased the channel activity of G551D-CFTR that had been already maximally potentiated by genistein, up to^-50% of the WT-CFTR level. The additive effects between curcumin and genistein not only support the hypothesis that multiple mechanisms are involved in the action of CFTR potentiators, but also suggests pharmaceutical relation between CFTR and ABCA3, because CFTR and ABCA3 are ABC protein family member and having NBD as a driving engine. Since primary culture of type II alveolar epithelial cells from the mouse, was able to made stable, we observed lamellar bodies in type II alveolar epithelial cells, using Coherent Anti-Stokes Raman Scattering(CARS) microscope. We could observe a lot of lamellar bodies expressing ABCA3, packing lipids to lamellar body and exocytosis to the outside of these cells.

在最大有效浓度下，姜黄素对G551D-CFTR全细胞和单通道电流的增加作用小于染料木素。然而，姜黄素进一步提高了G551D-CFTR的通道活性，达到WT-CFTR水平的^-50%，而G551D-CFTR已经被染料木素最大限度地增强了。姜黄素与染料木素的叠加效应不仅支持了CFTR增强剂作用机制多样的假说，而且还提示了CFTR与ABCA3之间的药物关系，因为CFTR和ABCA3都是ABC蛋白家族成员，并以NBD为驱动引擎。由于小鼠II型肺泡上皮细胞的原代培养能够稳定，我们使用相干抗斯托克斯拉曼散射（CARS）显微镜观察了II型肺泡上皮细胞的片层体。我们可以观察到大量表达ABCA3的片层体，将脂质堆积到片层体中，并向这些细胞外分泌细胞。

项目成果

Blocking kinetics of CFTR channel by aromatic carboxylic acid positionalisomers characterised using a novel amplitude distribution analysis method.

使用新型振幅分布分析方法表征芳香族羧酸位置异构体的 CFTR 通道阻断动力学。

• 发表时间: 2010
• 作者: Yu YC;Matsuzaki Y;et al.(8名中5番目)
• 通讯作者: et al.(8名中5番目)

Additive potentiating effects of genistein and curcumin on an ABC transporter CFTR with a disease-associated mutation.

金雀异黄素和姜黄素对具有疾病相关突变的 ABC 转运蛋白 CFTR 的附加增强作用。

• 发表时间: 2011
• 作者: Yu YC;Miki H;Matsuzaki Y;et al
• 通讯作者: et al

正期産児・早産児における胃液中肺サーファクタント蛋白A濃度の検討

足月儿和早产儿胃液肺表面活性蛋白A浓度的检测

• 发表时间: 2010
• 作者: 松崎陽平;et al
• 通讯作者: et al

Effects of aromatic carboxylic acids on genistein- and curcumin- potentiated G551D-CFTR.

芳香族羧酸对金雀异黄素和姜黄素增强的 G551D-CFTR 的影响。

• 发表时间: 2010
• 作者: Nakamura Y;Matsuzaki Y;et al.(10名中5番目)
• 通讯作者: et al.(10名中5番目)

Surfactant protein A in gastric fluid at birth as a useful marker of differentiation diagnosis between respiratory distress syndrome and transient tachypnea of the newborn

出生时胃液中的表面活性蛋白A作为新生儿呼吸窘迫综合征和短暂性呼吸急促鉴别诊断的有用标志物

• DOI: 10.1111/j.1442-200x.2011.03440.x
• 发表时间: 2011
• 期刊: Pediatr Int
• 影响因子: 1.4
• 作者: Matsuzaki Y;Hokuto I;Ikeda K
• 通讯作者: Ikeda K