Development of treatment of harlequin ichthyosis model mouse using ABCA12-reconstruction liposome

ABCA12重建脂质体治疗丑角鱼鳞病模型小鼠的研究进展

• 批准号: 21791053
• 负责人: YAMANE Naoko
• 金额: $ 2.75万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791053/
• 关键词: ABCA12; 遺伝性角化症; 脂質輸送

Recently, it was reported that ABCA12 works as an epidermal keratinocyte lipid transporter and that defective ABCA12 results in a loss of the skin lipid barrier, leading to Harlequin Ichthyosis. Until now, the mainstream of the treatments of hereditary keratosis were supportive measures. The development of the treatment method along the pathophysiology of HI is hoped by analyzing the function of ABCA12 more in detail. In this study, to prepare recombinant ABCA12, its expression vector was transfected to mammalian cells using liposomes. Moreover, we aimed to establish the stable cell line of which the expression of ABCA12 is suppressed, and the improvement of the lipid transport by the ABCA12 replacement is examined. It would be interesting to further examine improvement of lipid transport by supplementation of ABCA12 using liposomes.

最近，有报道称ABCA12作为表皮角质形成细胞脂质转运体，ABCA12缺陷导致皮肤脂质屏障丧失，导致丑角鱼鳞病。到目前为止，遗传性角化病的主流治疗方法是支持性措施。更详细地分析ABCA12的功能，希望能沿着HI的病理生理发展治疗方法。本研究通过脂质体将重组ABCA12表达载体转染到哺乳动物细胞中制备重组ABCA12。此外，我们旨在建立抑制ABCA12表达的稳定细胞系，并检测ABCA12替代对脂质转运的改善作用。通过脂质体补充ABCA12对脂质转运的改善将是一个有趣的进一步研究。

项目成果

低用量エトポシド内服療法が有効であったAnaplastic large cell lymphomaの1例

小剂量依托泊苷口服治疗间变性大细胞淋巴瘤有效一例

• 发表时间: 2009
• 期刊: Skin Cancer 24(1)
• 作者: 山根尚子;加藤直子;柳輝希;大澤倫子;西村真智子;伊藤幹;斎藤奈央
• 通讯作者: 斎藤奈央

Generalized exacerbation of systemic allergic dermatitis due to zinc patch test and dental treatments.

由于锌斑贴试验和牙科治疗导致全身过敏性皮炎普遍恶化。

• 发表时间: 2010
• 期刊: Contact Dermatitis 62
• 作者: Saito N;Yamane N;Matsumura W;Fujita Y;Inokuma D;Kuroshima S;Hamasaka K;Shimizu H.
• 通讯作者: Shimizu H.

【lichen】臨床例 慢性C型肝炎に伴った粘液水腫性苔癬

[地衣]临床病例：慢性丙型肝炎相关粘液水肿性地衣

• 发表时间: 2010
• 期刊: 皮膚病診療
• 作者: 山根尚子;他
• 通讯作者: 他

Pyoderma Gangrenosum of the Eyelid : Report of Two Cases and Revie of the Literature.

眼睑坏疽性脓皮病二例报告及文献复习。

• 发表时间: 2010
• 期刊: Dermatology
• 影响因子: 3.4
• 作者: Saito N;Yanagi T;Akiyama M;Yamane N;et al
• 通讯作者: et al

低用量エトポシド内服療法が有効であった anaplastic large cell lymphoma の1例

小剂量依托泊苷口服治疗有效的间变性大细胞淋巴瘤一例

• 发表时间: 2009
• 期刊: Skin Cancer 24巻
• 作者: 山根尚子;他
• 通讯作者: 他