Systematic analysis of cornification related molecules and development of new treatments for skin disorders.
Systematic analysis of cornification related molecules and development of new treatments for skin disorders.
批准号:
21791066
负责人:
MAKINO Teruhiko
金额:
$2.66万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
Profilaggrin n端(proFLG-N)由两个不同的结构域组成,一个类似s100的a结构域和B结构域。在NHK的生长期转染proFLG-N后,只有表达proFLG-N的细胞表现出DNA降解。因此,proFLG-N中的a结构域负责DNA降解。这些结果表明,proFLG-N在角化细胞终末分化,特别是去核过程中起重要作用。另一方面,我们鉴定了新的S100融合型蛋白,聚丝蛋白-2(FLG2)和毛透明蛋白样蛋白1(TCHHL1)。FLG2的结构特征和表达谱与profilaggrin相似。chchl1的氨基酸序列包含一个跨膜结构域。chchl1蛋白在基底层表达。
英文摘要
The Profilaggrin N-terminal(proFLG-N) consists of two distinct domains, an S100-like A-domain and B domain. After proFLG-N transfection at a growing phase of NHK, only proFLG-N expressing cells exhibited DNA degradation. Therefore, A-domain in proFLG-N is responsible for DNA degradation. These results indicate that proFLG-N play an important role in the keratinocyte terminal differentiation, especially in the denucleation process. On the other hand, we identified novel S100 fused-type proteins, filaggrin-2(FLG2) and trichohyalin-like protein 1(TCHHL1). The structural features and expression profile of FLG2 were similar to those of profilaggrin. The deduced amino acid sequence of TCHHL1 contains one trans-membrane domain. The TCHHL1 protein was expressed in the basal layer.
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An ultrastructual examionation of a blistering lesion of mycosis fungoides bullosa
大疱蕈样肉芽肿起泡病灶的超微结构检查
DOI:
10.1111/j.1365-2133.2011.10323.x
发表时间:
2011
期刊:
Br.J.Dermatol.
影响因子:
--
作者:
[Ueda C., Makino T., et. al]
通讯作者:
et. al
Ultraviolet B irradiation induces the expression of hornein, a member of the S100 fused-type protein family in human skin.
紫外线 B 照射可诱导人体皮肤中 S100 融合型蛋白家族成员霍氨酸的表达。
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Makino T., Yamakoshi T., Furuichi M., Huh NH., Shimizu T.]
通讯作者:
Shimizu T.
アトピー性皮膚炎をどのように診るか
如何诊断特应性皮炎
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Makino T, et al., 牧野輝彦]
通讯作者:
牧野輝彦
Involvement of multiple pathways in keratinocyte DNA degradation
多种途径参与角质形成细胞 DNA 降解
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Yamamoto M., Makino T., Motoyama A., Tsuboi R. and Hibino T.]
通讯作者:
Tsuboi R. and Hibino T.
Detection of antibodies against epidermal transglutaminase but not tissue transglutaminase in Japanese patients with dermatitis herpetiformis
日本疱疹样皮炎患者检测到表皮转谷氨酰胺酶抗体,但未检测到组织转谷氨酰胺酶抗体
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Makino T., Ishida W., Furuichi M., and Shimizu T.]
通讯作者:
and Shimizu T.
共 32 条
Analysis of molecular mechanism in keratinocyte terminal differentiation
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批准号:24591643
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.41万
-
财政年份:2012
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负责人:MAKINO Teruhiko
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依托单位:
Functional analysis of human hornerin and its contribution to human skin disdrders
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批准号:19790778
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.36万
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财政年份:2007
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负责人:MAKINO Teruhiko
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依托单位:
海外基金