Analysis of allergen-specific IgE repertoire in wheat allergy and its application for the diagnosis

点击翻译按钮获取中文摘要

In this study, we tried to produce the recombinant wheat allergens in Escherichia coli to establish a new in vitro method for accurate diagnosis of wheat allergy. We succeeded in producing the water-soluble wheat allergens as fusion proteins with glutathione-S-transferase by means of a co-expression of chaperon plasmid and pGEX-6p-1. The recombinant water-insoluble allergens were expressed using a pET or pCold system. The IgE-binding to some of the recombinant allergens were detected indicating that these recombinant proteins can be applied to the measuring of allergen-specific IgE antibodies.