Roles of sphingosine-1-phosphate foreosinophil localization and function in mice

1-磷酸鞘氨醇前嗜酸性粒细胞定位和功能在小鼠中的作用

• 批准号: 21791105
• 负责人: SUGITA Kazunari
• 金额: $ 2.66万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791105/
• 关键词: 皮膚炎症; 再生学; 好酸球; S1P; S1P1; FTY720

We monitored numerical alternations of eosinophils in IL-5 transgenic mice following FTY720 administration. The number of circulating eosinophils was significantly decreased in IL-5 transgenic mice treated by FTY720,suggesting that eosinophils are S1P-S1P1 sensitive. Accordingly, eosinophils showed a chemotactic response to S1P through a transwell assay. Additionally,eosinophils accumulated in the bone marrow, when S1P mediated signaling was disrupted by FTY720 treatment. We then showed the increment of cutaneous eosinophils using a murine atopic dermatitis model induced by repeated hapten application. FTY720 administration before the final challenge decreased the number of cutaneous eosinophils along with reduction of the ear swelling response, of the late phase reaction. These findings suggest that S1P-S1P1 signaling regulates the egress of eosinophils from the bone marrow and affects cutaneous eosinophil localization and resultant late phase reaction.

我们监测了FTY 720给药后IL-5转基因小鼠中嗜酸性粒细胞的数量变化。经FTY 720处理的IL-5转基因小鼠的循环嗜酸性粒细胞数量显著减少，表明嗜酸性粒细胞对S1 P-S1 P1敏感。因此，嗜酸性粒细胞通过transwell试验显示出对S1 P的趋化反应。此外，当FTY 720处理破坏S1 P介导的信号传导时，嗜酸性粒细胞在骨髓中积累。然后，我们显示了皮肤嗜酸性粒细胞的增量使用小鼠异位性皮炎模型诱导重复应用半抗原。在最后一次激发前给予FTY 720可降低皮肤嗜酸性粒细胞的数量，沿着耳肿胀反应和晚期反应的降低。这些发现表明，S1 P-S1 P1信号调节嗜酸性粒细胞从骨髓的出口，并影响皮肤嗜酸性粒细胞的定位和由此产生的晚期反应。

项目成果

Sphingosine-1phosphate regulates cutaneous localization and bone marrow egress of eosinophils.

1 磷酸鞘氨醇调节嗜酸性粒细胞的皮肤定位和骨髓流出。

• 发表时间: 2009
• 作者: Sugita K;Kabashima K;Sakabe J;Yoshiki R;Tanizaki H;Tokura Y.;Kazunari Sugita;杉田和成;杉田和成;杉田和成
• 通讯作者: 杉田和成

FTY720 regulates bone marrow egress of eosinophils and modulates late-phase skin reaction in mice.

FTY720 调节嗜酸性粒细胞的骨髓流出并调节小鼠的后期皮肤反应。

• 发表时间: 2010
• 期刊: Am J Pathol. 177
• 作者: Sugita K;Kabashima K;Sakabe J;Yoshiki R;Tanizaki H;Tokura Y.
• 通讯作者: Tokura Y.

FTY720 regulates bone marrow egress of eosinophils and modulates late-phase skin reaction in mice

FTY720 调节小鼠嗜酸性粒细胞的骨髓流出并调节后期皮肤反应

• 发表时间: 2010
• 期刊: The American Journal of Pathology
• 作者: Sugita K;Kabashima K;Sakabe J;Yoshiki R;Tanizaki H;Tokura Y.;Kazunari Sugita
• 通讯作者: Kazunari Sugita

Sphingosine-1phosphate regulates cutaneous localization and bonemarrow egress of eosinophils.

1 磷酸鞘氨醇调节嗜酸性粒细胞的皮肤定位和骨髓流出。

• 发表时间: 2009
• 作者: Sugita K;Kabashima K;Sakabe J;Yoshiki R;Tanizaki H;Tokura Y.;Kazunari Sugita;杉田和成
• 通讯作者: 杉田和成

Sphingosine-1phosphate regulates cutaneous localization and bone marrow egress of eosinophils

1 磷酸鞘氨醇调节嗜酸性粒细胞的皮肤定位和骨髓流出

• 发表时间: 2009
• 作者: Sugita K;Kabashima K;Sakabe J;Yoshiki R;Tanizaki H;Tokura Y.;Kazunari Sugita;杉田和成;杉田和成
• 通讯作者: 杉田和成