Probing the mechanism of reduced serotonin transporter densities in autistic brains : a postmortem brain study

探讨自闭症大脑中血清素转运蛋白密度降低的机制：死后大脑研究

• 批准号: 21791125
• 负责人: SUDA Shiro
• 金额: $ 2.75万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791125/
• 关键词: 児童; 思春期精神医学; 自閉症; 死後脳研究; セロトニン神経; トランスポーター; 蛋白発現解析

Emerging evidence from neuroimaging study using positron emission tomography (PET) suggests reduced serotonin transporter (SERT) densities in the brains of subjects with autism. To probe the mechanism of reduced SERT densities in autistic brains、we examined the postmortem brains of autistic subjects to identify any changes in the expression of SERT proteins. mRNA and protein expression of SERT protein were examined in the autistic brain and control subjects using real time PCR and Western blotting. The result showed that none of mRNA and protein expression was found to be significantly altered. Since PET tracers bind the membrane expressed SERT proteins, this finding suggests that dysregulation of protein transport, trafficking and transporter internalizing systems may underlie the reduced SERT densities in autistic brains.

使用正电子发射断层扫描 (PET) 进行的神经影像学研究的新证据表明，自闭症患者大脑中的血清素转运蛋白 (SERT) 密度降低。为了探究自闭症患者大脑中 SERT 密度降低的机制，我们检查了自闭症受试者死后的大脑，以确定 SERT 蛋白表达的任何变化。使用实时 PCR 和蛋白质印迹法检查自闭症大脑和对照受试者中 SERT 蛋白的 mRNA 和蛋白表达。结果显示，mRNA和蛋白质表达均未发现显着改变。由于 PET 示踪剂与膜表达的 SERT 蛋白结合，这一发现表明蛋白质转运、运输和转运内化系统的失调可能是自闭症大脑中 SERT 密度降低的原因。

项目成果

Handbook of Behavior, Food and Nutrition(Disturbed growth in early life and later neurocognitive development associated with psychiatric disorders.)(Preedy VR, Watson RR, Martin CR (Eds.))chapter 100 New York

行为、食物和营养手册（生命早期的生长障碍和后来与精神疾病相关的神经认知发育。）（Preedy VR，Watson RR，Martin CR（编辑））第 100 章纽约

• 发表时间: 2011
• 作者: Suda S;Takei N.
• 通讯作者: Takei N.

Donepezil and concurrent sertraline treatment is associated with increased volume of the hippocampi in a patient with depression

多奈哌齐和舍曲林同时治疗与抑郁症患者海马体积增加有关

• 发表时间: 2010
• 期刊: Journal of Clinical Psychiatry (印刷中)
• 作者: Suda S;et al
• 通讯作者: et al

Searching for very early precursors of autism spectrum disorders : the Hamamatsu Birth Cohort for Mothers and Children (HBC).

寻找自闭症谱系障碍的早期先兆：滨松母亲和儿童出生队列 (HBC)。

• 发表时间: 2010
• 期刊: Journal of Developmental Origins of Health and Disease 1
• 作者: Tsuchiya KJ;Matsumoto K;Suda S;Miyachi T;Itoh H;Kanayama N;Hirano K;Ohzeki T;Takei N;the HBC Study Team.
• 通讯作者: the HBC Study Team.

Dysregulation of glutamatergic biosynthetic pathway in subjects with autism.

自闭症患者谷氨酸生物合成途径失调。

• 发表时间: 2010
• 作者: Shimmura C;Suda S;et al
• 通讯作者: et al

Dysregulation of glutamatergic biosynthetic pathway in subjects with autism.Neuroscience 2010

自闭症患者谷氨酸生物合成途径失调。神经科学 2010

• 发表时间: 2010
• 作者: Shimmura C;Suda S;Tsuchiya KJ;Hashimoto K;Matsuzaki H;Iwata Y;Kameno Y;Nakamura K;Takei N;Mori N.
• 通讯作者: Mori N.