Establishment of tracking method for induced pluripotent stem cells using 64-Cu positron emitting isotope

64-Cu正电子发射同位素标记诱导多能干细胞（iPSCs）是一项技术成功，使用PTSM材料作为结合材料。并成功获得了iPSC分布的正电子发射断层扫描（PET）图像。采用了两种给药途径；1)经尾静脉静脉注射，2)左心室直接注射。各分布之间存在显著差异。静脉注射仅见肺分布。另一方面，描述了脑、肾和移植肿瘤的分布。由于64-Cu的半衰期为13小时，因此在标记细胞注射后24小时即可获得PET图像。与18F-FDG标记的iPSCs图像比较，64-Cu标记的iPSCs在正电子标记细胞给药后6小时内的图像信噪比低于18F-FDG。

Induced pluripotent stem cells (iPSCs) Labeling by 64-Cu positron emitting isotopes is a technical success with using PTSM material as a binding material. And positron emission tomography (PET) images which indicate iPSC distributions were acquired successfully. Two administration pathways were used ; 1) intravenous injection via tail vein, 2) left ventricle injection directly. There was significant difference between each distribution. Only lung distribution was seen by intravenous injection. On the other hand, the distributions to brain, kidneys, and transplanted tumors were described. Since half-life for 64-Cu is 13 hours, PET images could be acquired at 24 hours after labeled cells injection. Comparison with images for 18F-FDG labeled iPSCs, 64-Cu labeled image has less signal-to-noise ratio than that of 18F-FDG within 6 hour after positron-labeled cells administration.