In situ analysis of crossbridge dynamics in failing heart model of rat using X-ray technique

这项研究的目的是发展一种方法，通过使用Spring-8观察分子纳米级的动力学来诊断心力衰竭。我们特别关注了心肌收缩蛋白，分析了肌动蛋白-肌球蛋白相互作用和肌球蛋白细丝之间的晶格间距，采用保持复杂形态和多重代偿机制的大鼠和心力衰竭模型。用异丙肾上腺素建立肥厚心脏模型，分析了舒张期和缩缩期以及肥厚心脏的横桥动力学，并对心力衰竭进行了评估。

This study was aimed for the development of the method to give heart failure a diagnosis by observing dynamics of the molecule nano-order using SPring-8. We paid attention to particularly myocardial contractile protein and analyzed actin-myosin interaction and lattice spacing between myosin filaments used the rats which maintained complicated form and a multiplex compensation mechanism and the heart failure model.We performed the measurement of the hypertrophic heart model by isoproterenol, analyzed crossbridge dynamics in the diastolic phase and the contraction phase and of the hypertrophic heart and evaluated the failing heart.