The molecular mechanisms of Perlecan for vascular invasion in growth plate development

Perlecan在生长板发育过程中血管侵袭的分子机制

• 批准号: 21791418
• 负责人: ISHIJIMA Muneaki
• 金额: $ 2.75万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791418/
• 关键词: 骨・軟骨代謝学; パールカン; ヘパラン硫酸プロテオグリカン; VEGF; 血管侵入; 内軟骨性骨化

Perlecan (Perl) is a heparin sulfate proteoglycan required for cartilage development. This study investigated the role of Perl for vascular invasion in growth plate development.While the vascular invasion was inhibited, VEGF expression was increased in Perl-/- growth plate. In organ culture experiment, the growth plate was expanded by the addition of FGF-FGFR inhibitor even though the absence of Perl.These data indicate that Perl is essential for vascular invasion in growth plate development by not only modulating FGF signaling but also regulating VEGF function.

Perlecan （Perl）是软骨发育所需的硫酸肝素蛋白多糖。本研究探讨了Perl在生长板发育过程中血管侵袭的作用。在抑制血管侵袭的同时，在Perl-/-生长板中VEGF表达增加。在器官培养实验中，在没有Perl的情况下，通过添加FGF-FGFR抑制剂使生长板扩大。这些数据表明，Perl不仅通过调节FGF信号传导，还通过调节VEGF功能，对生长板发育过程中的血管侵袭至关重要。