The molecular mechanisms of Perlecan for vascular invasion in growth plate development

Perlecan(Perl)是一种软骨发育所需的硫酸肝素蛋白多糖。本研究探讨了Perl对血管侵袭在生长板发育中的作用。在抑制血管侵袭的同时，Perl-/-生长板中血管内皮生长因子的表达增加。在器官培养实验中，即使在没有Perl的情况下，添加成纤维细胞生长因子-FGFR抑制剂也可以使生长板扩张。这些数据表明，Perl不仅通过调节成纤维细胞生长因子信号通路，而且还通过调节血管内皮生长因子的功能，在生长板的发育过程中对血管的侵袭起着至关重要的作用。

Perlecan (Perl) is a heparin sulfate proteoglycan required for cartilage development. This study investigated the role of Perl for vascular invasion in growth plate development.While the vascular invasion was inhibited, VEGF expression was increased in Perl-/- growth plate. In organ culture experiment, the growth plate was expanded by the addition of FGF-FGFR inhibitor even though the absence of Perl.These data indicate that Perl is essential for vascular invasion in growth plate development by not only modulating FGF signaling but also regulating VEGF function.