Development of new gene knockdown techniques for platelets using miRNA

将miR RNAi表达载体导入人CD34+祖细胞和小鼠骨髓细胞中，体外培养3-4周后分化为血小板，证实了目的基因在血小板细胞中被敲除。这些血小板的聚集能力和P-选择素表达对激动剂的反应较弱。通过注射这些基因击倒的血小板，小鼠实验模型的存活率和肺栓塞的严重程度都有所改善。有必要进行进一步的研究，进行详细的分析。

After transfection of miR RNAi expression vector in human CD34+ progenitor cells and mouse bone marrow cells along with differentiating into platelets with 3-4week culture in vitro, we have confirmed the target gene knocked down in platelet cells. These platelets have less ability of aggregation and P-selectin expression in response to agonists. With injection of these gene knocked down platelets, survival and severity of pulmonary embolism have improved in mouse experimental model. Further studies are necessary to undergo detailed analyses.