Development of new gene knockdown techniques for platelets using miRNA

使用 miRNA 开发血小板新基因敲除技术

• 批准号: 21791466
• 负责人: KATO Yuko
• 金额: $ 2.83万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791466/
• 关键词: 周術期管理学; 遺伝子治療; 血小板

After transfection of miR RNAi expression vector in human CD34+ progenitor cells and mouse bone marrow cells along with differentiating into platelets with 3-4week culture in vitro, we have confirmed the target gene knocked down in platelet cells. These platelets have less ability of aggregation and P-selectin expression in response to agonists. With injection of these gene knocked down platelets, survival and severity of pulmonary embolism have improved in mouse experimental model. Further studies are necessary to undergo detailed analyses.

将miR RNAi表达载体转染人CD34+祖细胞和小鼠骨髓细胞，体外培养3-4周后分化成血小板，证实了靶基因在血小板细胞中被敲低。这些血小板对激动剂的聚集能力和p选择素表达能力较弱。在小鼠实验模型中，注射这些基因敲除的血小板可改善肺栓塞的存活率和严重程度。进一步的研究需要进行详细的分析。

项目成果

Transillumination by light-emitting diode facilitates peripheral venous cannulations in infants and small children.

发光二极管透照有利于婴儿和幼儿的外周静脉插管。

• 发表时间: 2010
• 期刊: Acta Anaesthesiol Scand. 54
• 作者: Hosokawa K;Kato H;Kishi C;Kato Y;Shime N.
• 通讯作者: Shime N.